13C代謝流和定量代謝物組學研究團隊
利用本實驗室先進的微型反應器、快速取樣裝置和高效液相/氣相質譜儀可以同時實現胞內代謝物濃度的測定和13C代謝流的計算，從而為以13C代謝流為核心的縱向組學整合技術打下堅實基礎。并與宏觀過程生物生理代謝參數OUR、CER、RQ等相結合分析，實驗發酵過程的全局優化。
快速取樣、淬滅和提取裝置示意圖
13C代謝流和定量代謝物組測定流程圖
13C代謝流和定量代謝物組學在黑曲霉生產糖化酶過程中的應用
研究成果
1.Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via13C metabolic flux analysis. Journal of Biotechnology, 2014, 187: 124-134.
2.Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger. Microbial Cell Factories, 2015, 14: 147-160.
3.Enhanced L-lactic acid production in Lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis. Applied Microbiology and Biotechnology, 2016, 100: 2301-2310.